81a stain example for stereology full#

Most of the morphometric parameters evaluated in H. The number of Sertoli cells per testis and per gram of testis was 7.8Г—10(6) and 95.28Г—10(6), respectively.

81a stain example for stereology full#

The mitotic and meiotic indexes showed losses of 85 and 42%, respectively and an overall loss of 90% over the full spermatogenic process. megacephalus presents 5.06 m of seminiferous tubules and an average of 27.96 m of seminiferous tubules per gram of testis. The diameter of the seminiferous tubules was 206.5 Ојm and the height of seminiferous epithelium was 71.27 Ојm. The volume density and volume of the interstitium were 5.54% and 0.011 mL, respectively. The volume density of the seminiferous tubules was 94.46%, which represented a volume of 0.18 mL. These parameters suggest promiscuous reproductive behavior, of the polyandrous type, favoring males with higher sperm production and consequently, larger testes. megacephalus in the study was 47.84 g, of which, 0.40% is allocated to the gonads (GSI) and 0.36% to the seminiferous tubules (TSI). The average body weight observed in the specimens of H. Images of the testicular parenchyma were obtained from photomicroscope and morphometric and stereological analyses were carried out using the Image Pro-Plus software. Two-micrometer-thick sections from each sample were stained with toluidine blue/sodium borate 1%. The testes were fixed in Karnovsky solution, dehydrated, and embedded in methacrylate. We used five animals in the study, captured in forest fragments in southwestern Goias State, between April and August 2009. This study aimed to assess the testis morphometry and stereology of Hylaeamys megacephalus. Information on reproductive characteristics of wild rodents is scarce in the literature.